Источник
Proceedings of the National Academy of Sciences RSS feed -- current issue
Выбор редакции
12 июня, 19:36

Transcriptional signature of the decidua in preeclampsia [Biological Sciences]

  • 0

An emerging concept is that the genesis of preeclampsia is rooted in dysregulated decidualization (1). In PNAS, Garrido-Gomez et al. (2) demonstrate a distinct transcriptional signature in the endometrial stromal cells (ESCs) of preeclampsia patients compared with control cells during in vitro decidualization (before pregnancy) and in vivo decidualization (after...

Выбор редакции
12 июня, 19:36

Electronic cigarette aerosols induce DNA damage and reduce DNA repair: Consistency across species [Biological Sciences]

  • 0

We read with great interest the work of Lee et al. (1), in which the authors report that mice exposed to electronic cigarette aerosol have increased levels of DNA lesions and decreased DNA repair activity. This work sparked important discussion both in PNAS (2, 3) and science media (4). We...

Выбор редакции
12 июня, 19:36

Reply to Queimado et al.: E-cigarettes induce DNA damage and inhibit DNA repair in mice and human cells [Biological Sciences]

  • 0

We believe that Queimado et al. (1) may have inadvertently missed several key details presented in our recent PNAS publication (2). First, for the determination of nicotine effects on DNA adduct formation and DNA repair activity, the nicotine concentrations used for 1-h treatments of human lung and bladder epithelial cells...

Выбор редакции
12 июня, 19:36

Two-way mixed-effects methods for joint association analysis using both host and pathogen genomes [Statistics]

  • 0

Infectious diseases are often affected by specific pairings of hosts and pathogens and therefore by both of their genomes. The integration of a pair of genomes into genome-wide association mapping can provide an exquisitely detailed view of the genetic landscape of complex traits. We present a statistical method, ATOMM (Analysis...

Выбор редакции
12 июня, 19:36

Mapping an ancient city with a century of remotely sensed data [Anthropology]

  • 0

The rapidly growing global population places cultural heritage at great risk, and the encroachment of modern settlement on archaeological sites means that valuable information about how past societies worked and interacted with the environment is lost. To manage and mitigate these risks, we require knowledge about what has been lost...

Выбор редакции
12 июня, 19:36

Gene cluster conservation provides insight into cercosporin biosynthesis and extends production to the genus Colletotrichum [Agricultural Sciences]

  • 0

Species in the genus Cercospora cause economically devastating diseases in sugar beet, maize, rice, soy bean, and other major food crops. Here, we sequenced the genome of the sugar beet pathogen Cercospora beticola and found it encodes 63 putative secondary metabolite gene clusters, including the cercosporin toxin biosynthesis (CTB) cluster....

Выбор редакции
12 июня, 19:36

Potent and selective antitumor activity of a T cell-engaging bispecific antibody targeting a membrane-proximal epitope of ROR1 [Applied Biological Sciences]

  • 0

T cell-engaging bispecific antibodies (biAbs) present a promising strategy for cancer immunotherapy, and numerous bispecific formats have been developed for retargeting cytolytic T cells toward tumor cells. To explore the therapeutic utility of T cell-engaging biAbs targeting the receptor tyrosine kinase ROR1, which is expressed by tumor cells of various...

Выбор редакции
12 июня, 19:36

In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking [Biochemistry]

  • 0

Covalently cross-linked pilus polymers displayed on the cell surface of Gram-positive bacteria are assembled by class C sortase enzymes. These pilus-specific transpeptidases located on the bacterial membrane catalyze a two-step protein ligation reaction, first cleaving the LPXTG motif of one pilin protomer to form an acyl-enzyme intermediate and then joining...

Выбор редакции
12 июня, 19:36

Sequential activation of human signal recognition particle by the ribosome and signal sequence drives efficient protein targeting [Biochemistry]

  • 0

Signal recognition particle (SRP) is a universally conserved targeting machine that mediates the targeted delivery of ∼30% of the proteome. The molecular mechanism by which eukaryotic SRP achieves efficient and selective protein targeting remains elusive. Here, we describe quantitative analyses of completely reconstituted human SRP (hSRP) and SRP receptor (SR)....

Выбор редакции
12 июня, 19:36

Protein kinase C{alpha} gain-of-function variant in Alzheimer’s disease displays enhanced catalysis by a mechanism that evades down-regulation [Biochemistry]

  • 0

Conventional protein kinase C (PKC) family members are reversibly activated by binding to the second messengers Ca2+ and diacylglycerol, events that break autoinhibitory constraints to allow the enzyme to adopt an active, but degradation-sensitive, conformation. Perturbing these autoinhibitory constraints, resulting in protein destabilization, is one of many mechanisms by which...

Выбор редакции
12 июня, 19:36

Structural basis for MTR4-ZCCHC8 interactions that stimulate the MTR4 helicase in the nuclear exosome-targeting complex [Biochemistry]

  • 0

The nuclear exosome-targeting (NEXT) complex functions as an RNA exosome cofactor and is involved in surveillance and turnover of aberrant transcripts and noncoding RNAs. NEXT is a ternary complex composed of the RNA-binding protein RBM7, the scaffold zinc-knuckle protein ZCCHC8, and the helicase MTR4. While RNA interactions with RBM7 are...

Выбор редакции
12 июня, 19:36

Dynamic action potential clamp predicts functional separation in mild familial and severe de novo forms of SCN2A epilepsy [Biophysics and Computational Biology]

  • 0

De novo variants in SCN2A developmental and epileptic encephalopathy (DEE) show distinctive genotype–phenotype correlations. The two most recurrent SCN2A variants in DEE, R1882Q and R853Q, are associated with different ages and seizure types at onset. R1882Q presents on day 1 of life with focal seizures, while infantile spasms is the...